Jinhua Shao1,2,
Yufei Zhang3,
Zhiyong Zhu1,2,
Xiaoming Chen1,2,
Fulin He1,2 
For correspondence:- Fulin He Email: il0749@163.com Tel:+86-15575312542
Accepted: 29 April 2018 Published: 28 May 2018
Citation: Shao J, Zhang Y, Zhu Z, Chen X, He F. Process optimization and insecticidal activity of alkaloids from the root bark of Catalpa ovata G. Don by response surface methodology. Trop J Pharm Res 2018; 17(5):843-848 doi: 10.4314/tjpr.v17i5.14
© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To optimize the extraction of total alkaloids from the root bark of Catalpa ovata using response surface methodology, and to determine the insecticidal activity of the total alkaloids extracted.
Methods: A combination of Box-Behnken design and response surface methodology (RSM) was used to optimize the acid water extraction of total alkaloids from the root bark of Catalpa ovata, with extraction rate of total alkaloids as index and the single factor experiment as basis, and the extraction time, material: liquid ratio and pH as 3 factors. The insecticidal activity of total alkaloids was determined against the three instar armyworm Mythimna separata (Lepidoptera: Noctuidae) and diamondback moth Plutella xylostella (Lepidoptera: Plutellidae).
Results: The optimum extraction conditions for total alkaloids were: material: liquid ratio of 1:10; extraction time of 3 h, pH of 1.0, and simmering. Under these conditions, total alkaloid extraction was 8.62 %, which was very close to the experimental value. The results were accurate and reliable, with reference value. The insecticidal activity of the total alkaloids indicate that when the concentration of the total alkaloids was 10 mg/L, the fatality rate of Plutella xyllostella and oriental armyworm was over 89 %, but the insecticidal activity of the total alkaloids was lower than that of avermectin which was employed as a reference.
Conclusion: The alkaloids from the root bark of Catalpa ovata are potential botanical insecticides.
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